Application

DILUTION:

Suggested dilution for most applications: 1:50-1:200

Optimal working dilutions must be determined by the end user.

Detect Goat IgG using this Rabbit anti-Goat IgG Antibody, FITC conjugate validated for use in IF.

Immunogen

Purified Goat IgG.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Liquid in 0.01 M Phosphate buffer, 0.25 M NaCl pH 7.6 with 15 mg/mL BSA, 0.01% Thimerosal and 0.05% sodium azide.

RECONSTITUTION:

Reconstitute to 1 mg/mL with sterile distilled water.

Specificity

Goat IgG (H&L)

FLUOROPHORE/PROTEIN:

Approximately 7.2 mg/mg; 2.7 moles FITC per mol IgG

Storage and Stability

Maintain lyophilized product between 2 and 8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.